Proximity-dependent biotinylation
Webb1 okt. 2024 · Using proximity-dependent biotinylation, followed by LC-MS/MS analysis and in silico phospho-site prediction, we identified 21 mitochondrial proteins potentially targeted by PKA. We confirmed the interaction of PKA with TIM44 using … Webb邻近标记是一种能够给与靶蛋白质瞬时靠近，或者互作（邻近）的蛋白质加上生物素的技术，它与质谱检测技术的联合使用能检测细胞过程中弱的、瞬时的蛋白质相互作用，有效解决上述问题。. 本文综述了基于生物素的邻近标记方法的发展现状，从依赖于融合 ...
Proximity-dependent biotinylation
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Webb28 juli 2024 · Proximity-dependent biotinylation approaches include biotin ligase-based methods such BioID () and peroxidase-based methods such as APEX () that permit the covalent labeling of preys proximal to a protein of interest in the context of living cells. Webb1 dec. 2016 · Proximity-dependent biotin identification (BioID) is quickly gaining popularity as a powerful tool for identifying novel protein-protein and proximity-based interactions in live cells. This technique relies on a promiscuous biotin ligase, which is fused to a …
Webb17 mars 2024 · Here we present a BioID-based map of a human cell based on 192 markers from 32 different subcellular compartments, comprising 35,902 high confidence proximity interactions, and defining the intracellular locations … We aimed to select at least three independent baits (referred to here as compartment markers) for all major membrane-bound and membraneless organelles in HEK293 cells, as well as for all cytoskeletal elements. For complex organelles, such as the nucleus and the mitochondrion, distinct markers were selected to … Visa mer No statistical methods were used to predetermine sample size. Mass spectrometry sample acquisition was randomized but no further experimental randomization was done. Investigators were blinded to … Visa mer HEK293 Flp-In T-REx cells were from Invitrogen and were authenticated by short-tandem repeat analysis with The Center for Applied Genomics Genetic Analysis Facility (Sick Kids Hospital, Toronto). HeLa cells … Visa mer The BirA* enzyme used in this study for profiling compartments was the original BioID enzyme previously described5. Two different BioID … Visa mer For BioID, the parental cell line, HEK293 Flp-In T-REx 293, was grown at 37 °C in DMEM high-glucose medium supplemented with 5% fetal bovine serum, 5% cosmic calf serum and 100 U ml−1penicillin/streptomycin … Visa mer
WebbDoctoral student studying the phenotypic landscape of human induced pluripotent stem cells across healthy individuals. I'm interested in linking … Webb1 maj 2024 · Proximity-dependent biotinylation (PDB) approaches involve fusion of a bait with an enzyme. • BioID (biotin protein ligase) and APEX (peroxidase) are distinct enzymes used in PDB. • Past, present and future development and applications of PDB are …
WebbProximity-dependent biotinylation for identification of interacting proteins. Curr. Protoc. Cell Biol. 2016 Dec 1 (73): 17.19.1-17.19.12. All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications. Related …
Webb15 juli 2024 · A proximity-dependent biotinylation map of a human cell 人类细胞的近端生物素酰化图谱 （导读 阿金）近端生物素酰化技术（BioID）有助于确定生物细胞中细胞区室组成。 本研究提供基于192个亚细胞标记物的人类细胞近端生物素酰化图谱，确定了HEK293细胞中4145种独特蛋白质的细胞内定位，在线粒体外膜和内质网界面处发现对 … col tim helfrichWebb24 feb. 2016 · The BioID method is based on proximity-dependent cellular biotinylation by a promiscuous bacterial biotin ligase ( Escherichia coli BirA R118G, hereafter called BioID; Choi-Rhee et al., 2004; Cronan, 2005) fused to a bait protein to generate a history of PPAs over time in living cells ( Roux et al., 2012 ). col. tim heinemannWebbIn this study, a fusion molecule of a recently developed proximity biotinylation enzyme (AirID) with rat CaM (AirID-CaM) was expressed and purified to near homogeneity using an E. coli expression system to examine the physical interactions between CaM and its target proteins by converting the interaction to biotinylation of CaM targets under … col tim hough usmcWebb11 apr. 2024 · PDF Proximity labeling (PL) coupled with mass spectrometry has emerged as a powerful technique to map proximal protein interactions in living cells ... col tim baileyWebbBiotinylation, also called biotin labeling, is most commonly performed through chemical means, although enzymatic methods are also available. Chemical methods provide greater flexibility in the type of biotinylation needed than enzymatic approaches and can be … dr theiss spray vitamin bWebb17 mars 2024 · Here we present a BioID-based map of a human cell based on 192 markers from 32 different subcellular compartments, comprising 35,902 high confidence proximity interactions, and defining the intracellular locations of 4,145 unique proteins in HEK 293 … col tim mundy usmcWebb29 sep. 2024 · Proximity-dependent biotinylation (PDB) is used as an interactome discovery method in living cells that involves the covalent modification of proteins with biotin based on their proximity to a protein of interest (Rees et al., 2015b; Roux, 2013; … col tim mitchell