SpletPlasmid pCW-Tet-Cas9-BFP-PGK-Blast from Dr. Bernhard Schmierer's lab contains the insert Cas9-T2A-TagBFP. This plasmid is available through Addgene. ... Vector Database. Digital collection of empty plasmid backbones from publications and commercially available sources. Help Center . Ordering & MTAs. Splet01. jan. 2013 · In the current protocol the pCW’ expression vector is used with NADPH-cytochrome P450 reductase coexpressed in a bicistronic format (see Note 2 ). Standard alkaline lysis preparations of plasmid DNA ( 17) are of sufficient quality for DNA shuffling. 2. Ultracompetent DH5αF’IQ™ strain Escherichia coli (Invitrogen, Carlsbad, CA) (see Note 3 …
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SpletAssociate the PCW file extension with the correct application. On. Windows Mac Linux iPhone Android. , right-click on any PCW file and then click "Open with" > "Choose another … SpletpACYC-GST Vector Source Constructed by Farrell MacKenzie Company Structural Genomics Consortium, Toronto Description pACYC-GST is a derivative of the pACYC-LIC+ vector (SGC) and contains the p15A origin, cat gene and argU, ileY and leuW tRNA genes, as well as lacI, T7 promoter and T7 terminator regions contained in pACYC-LIC+. The jcpenney port charlotte fl closing
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SpletOur aim is to develop an inducible lentiviral vector-based gene-editing system for C-X-C chemokine receptor 4 (CXCR4) by CRISPR/Cas9, and to demonstrate its function in MKN … SpletpCWori (Search Vector Database) Backbone size w/o insert (bp) 5000 Total vector size (bp) 6365 Vector type Bacterial Expression Growth in Bacteria Bacterial Resistance (s) … Splet01. sep. 1998 · Abstract. Human cytochrome P450 (P450) 1B1 was expressed in Escherichia coli at a level of 200 nmol/liter culture using a pCW vector by removal of … jcpenney poncho shrug